The game was released for PlayStation 4, Windows, and Xbox One on September 25, 2020, both individually and as part of the Mafia: Trilogy pack, which also includes a remastered version of the second game and a lightly altered port of the third game which includes its additional story packs. An original score was also created for the game.

While some of the veteran Czech Mafia voice cast returned for the remake, the English voices were recast. The gameplay builds upon 2016's Mafia III, and features enhanced mission dynamics and the introduction of motorcycles to the series. This is mostly done in the Free Ride game mode, where players are not restricted by the linear nature of missions and can find hidden side missions and collectibles exclusive to this mode. The game's open world nature allows players to explore Lost Heaven at their leisure when not completing missions to advance the narrative. Like the original game, the remake is set within the fictional city of Lost Heaven, Illinois, during the 1930s, and follows the rise and fall of Tommy Angelo, a Sicilian American cab driver-turned-gangster, within the Salieri crime family. It is a remake of the 2002 video game Mafia. Mafia: Definitive Edition is a 2020 action-adventure game developed by Hangar 13 and published by 2K.